Serial Dilution Calculator

Plan a dilution series, calculate transfer and diluent volumes, and prepare a table you can copy, download, or print for bench work.

Dilution series setup

Use one dilution factor for the whole series, or set a different factor for each tube.

The selected unit is carried through the series; no unit conversion is performed.
Up to 12 tubes.

Why serial dilution matters

A serial dilution makes a stepwise concentration series from one stock solution. It is useful for standard curves, plating assays, dose responses, enzyme tests, and any workflow where each tube is prepared from the previous one.

The calculator uses the selected dilution factor to compute the transfer volume and diluent volume for every tube. For a 1:10 dilution with a 1 mL final volume, each tube uses 0.1 mL sample and 0.9 mL diluent.