20X SSPE Buffer Recipe

ComponentAmount20X StockFinal 1X
NaCl175.3 g3 M150 mM
NaH₂PO₄ · H₂O27.6 g200 mM10 mM
Na₂EDTA7.4 g200 mM10 mM

Dissolve the reagents in 800 ml ultrapure water. Adjust the pH to 7.4 with 10 M NaOH. Bring the volume to 1 liter with ultrapure water. Sterilize by autoclaving.

Good luck with your experiments!