10X TE Buffer Recipe (Tris-EDTA)

This buffer consists of 100 mM Tris-HCl (pH 7.5) and 10 mM EDTA (pH 8.0). Working concentration is 1X or lower.

Make it from a 1 M stock of Tris-HCl (pH 7.5) and a 500 mM stock of EDTA (pH 8.0). To make 1 liter of 10X TE (Tris-EDTA) buffer, mix the following:

• 100 ml 1 M Tris-HCl pH 7.5
• 20 ml 500 mM EDTA pH 8.0
• 880 ml ultrapure water

Good luck with your experiments!