Tris-HEPES-SDS Running Buffer (10X)

Recipe for 10X Tris-HEPES-SDS buffer:

• Tris base — 121 g
• HEPES — 238 g
• SDS — 10 g
• add ultrapure water to 1,000 ml

1X Tris-HEPES-SDS running buffer consists of:

• 100 mM Tris
• 100 mM HEPES
• 3 mM SDS

1X Tris-HEPES-SDS running buffer is used to run Precise Protein Gels (Pierce) and is compatible with Tris-glycine SDS sample loading buffer.

Good luck with your experiments!