TBE Buffer Recipe (Tris-Borate-EDTA)
10X TBE Buffer Preparation
Mix the following components to make 1 liter of 10X TBE.
| Component | Amount | 10X Stock | Final 1X |
|---|---|---|---|
| Tris base | 108 g | 890 mM | 89 mM |
| Boric acid | 55 g | 890 mM | 89 mM |
| EDTA (pH 8.0) | 40 ml | 20 mM | 2 mM |
5X TBE Buffer Preparation
Mix the following components to make 1 liter of 5X TBE.
| Component | Amount | 5X Stock | Final 1X |
|---|---|---|---|
| Tris base | 54 g | 445 mM | 89 mM |
| Boric acid | 27.5 g | 445 mM | 89 mM |
| EDTA (pH 8.0) | 20 ml | 10 mM | 2 mM |
Note: Filtering through a 0.22 micron filter delays precipitation; 10X TBE is more likely to precipitate. The pH of the stock solution will be around 8.3 — do not adjust the pH.
Good luck with your experiments!