TBS Buffer Recipe (Tris-Buffered Saline)

10X TBS: 500 mM Tris·HCl, pH 7.4 and 1500 mM NaCl.

1X TBS: 50 mM Tris·HCl, pH 7.4 and 150 mM NaCl.

To prepare 1000 ml of 10X TBS stock solution, mix the following reagents:

• 200 ml of ultrapure water
• 500 ml of 1 M Tris·Cl pH 7.4
• 300 ml of 5 M NaCl (or use 90 g of NaCl crystals)

Note: Tris concentration in 1X TBS varies from 20 mM to 100 mM from recipe to recipe. 50 mM is a commonly used concentration.

Good luck with your experiments!