TBST Buffer Recipe

1X TBST: 50 mM Tris·HCl, pH 7.4, 150 mM NaCl, 0.1% Tween 20.

To make 1000 ml of TBST buffer solution, mix the following reagents:

  • 100 ml 10X TBS
  • 10 ml 10% Tween 20
  • 890 ml ultrapure water

Good luck with your experiments!